Faculty Profile
Dr. Carrier Ewa Carrier, M.D.
Associate Professor of Medicine and Pediatrics
Division of Bone Marrow Transplantation
Office Address:
Division of Bone Marrow Transplantation
University of California, San Diego
Moores UCSD Cancer Center
3855 Health Sciences Drive, MC-0960
La Jolla, CA 92093
ecarrier@ucsd.edu
Office: (858) 822-6843
FAX: (858) 822-6844
| Education/Training | Research Interests | Publications | Laboratory |
Education and Training
| Pre-med | University Preparatory School, Poland | 1972-1976 |
| MD | Wroclaw University School of Medicine, Wroclaw, Poland | 1976-1982 |
| Internship | General Hospital, Jelenia Gora, Poland | 1982-1983 |
| Residency (Neurology) | General Hospital, Jelenia Gora, Poland | 1983-1984 |
| Residency (Pediatrics) | White Memorial Medical Center, Los Angeles, CA | 1987-1990 |
| Residency (Pediatrics) | Stanford University Medical Center, Stanford, CA | 1990-1991 |
| Fellowship (Hematology/Oncology) | Children’s Hospital, Oakland, CA | 1991-1994 |
| Fellowship (Pediatric Transplant Program) | University of California, San Francisco, CA | 1994-1887 |
Research Interests
Differentiation of human embryonic stem cells (ES) into hematopoietic progenitors and animal models of in vivo hematopoiesis and stem cell repair. The main interest of our laboratory is in vitro differentiation of human embryonic stem cells into hematopoietic progenitors, with special emphasis on dual hematopoietic/endothelial precursor called hemangioblast. Our hypothesis is that human ES cells can readily differentiate into hematopoietic progenitors with the use of appropriate cytokines and that this process can be optimized and upscaled using bioscaffolds. We focus on studying molecular mechanisms and signaling pathways involved in this process. The ultimate goal is to develop in vitro generated, human ES-derived erythrocytes for transfusion medicine needs.
Publications
Carrier, E, Lee, TH, Busch, M and Cowan, MJ. Induction of tolerance in non-defective mice after in utero transplantation of major histocompatibility complex-mismatched fetal hematopoietic stem cells. Blood 86(12): 4681-4690, 1995.
Donahue J, Gilpin B, Lee, T.H., Busch, M.P., Croft, M., and Carrier E. Microchimerism does not induce tolerance and sustains immunity after in utero transplantation. Transplantation, Vol 71(3) 359-368 February, 2001.
Donahue J, Gilpin E, Young D. and Carrier, E. Postnatal cytokines and boosts improve chimerism and hematological parameters in β-thalassemic mice transplanted in utero. Transplantation, Vol 71:10 1491-1494, May 2001.
Francis, K., Palsson, B., Donahue, J., Fong, S., Carrier, E. Murine Sca-1+/Lin- cells and human KG1a cells exhibit multiple pseudopod morphologies during migration. Exp. Hematol. 30: 460-463, 2002.
Sefrioui H, Donahue J, Gilpin EA, Srivastava AS, Carrier E. Tolerance and immunity following in utero transplantation of allogeneic fetal liver cells: the cytokine shift. Cell Transplant. 2003; 12 (1): 75-82.
Sefrioui H, Donahue J, Srivastava AS, Gilpin E, Lee TH, Carrier E. Alloreactivity following in utero transplantation of cytokine-stimulated hematopoietic stem cells: the role of recipient CD4(-) cells. Exp Hematol. 2002 Jun; 30(6): 617-24.
Feng Z, Carrier E. A Regulatory Role of Wnt Signaling Pathway in the Hematopoietic Differentiation of Murine Embryonic Stem Cells. Biochem Biophys Res Commun. 2004 Nov 26; 324(4): 1333-9
Moustafa M, Srivastava A, Nedelcu E, Donahue J, Gueorguieva I, Shenouda S, Minev B, and Carrier E., Chimerism and tolerance post in utero transplantation with ontogenically different sources of stem cells. Transplantation. 2004 Nov 15; 78(9): 1274-82.
Srivastava AS, Shenouda S, Mishra R, Carrier E. Transplanted embryonic stem cells successfully survive, proliferate and migrate to damaged regions of the mouse brain. Stem Cells 2006 Jul;24(7):1689-94.
Srivastava AS, Kaushal S, Mishra R, Lane TA, Carrier E. Dexamethasone facilitates erythropoiesis in murine embryonic stem cells differentiating into hematopoietic cells in vitro. Biochem Biophys Res Commun. 2006 Jul 28; 346(2):508-16.
Anand S. Srivastava, Elena Nedelcu, Babak Esmaeli-Azad, Rangrath Mishra, Carrier E. Thrombopoietin enhances generation of CD34+ cells from human embryonic stem cells. Stem Cells 2007:25:1456-1461.
Srivastava AS, Feng Z, Mishra R, Malhotra R, Kim HS, Carrier E. Embryonic stem cells ameliorate piroxicam-induced colitis in IL10-/-KO mice. Biochem Biophys Res Commun. 2007 Oct 5; 361(4):953-9.
Srivastava AS, Malhotra R, Esmaeli-Azad B, Lane T, Carrier E. Prospects of embryonic stem cells in treatment of hematopoietic disorders. Curr Pharm Biotechnol. 2007 Oct 8(5):305-17.
Laboratory
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Laboratory Personnel
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| Name | Title | Laboratory Focus |
| Andre Alfaro | Student Helper | |
| Azad Babak, Ph.D. | Research Scientist | Dr. Babak developed unique, bioscaffold-based artificial niche. He is assisting in the development of artificial stem cell niche to upscale production of ES-derive Hematopoietic progenitors. |
| Boris Minev, MD | Research Scientist | Dr. Minev is involved in work related to the generation of hematopoietic progenitors from human ES cells in vitro with special emphasis on molecular pathways involved in this process and HLA-expression. |
| Sharon Wampler, Ph.D | Laboratory manager | Dr. Wampler serves as lab manager. |
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